The rumen-mimetic continuous cultivation system (RMS) was used to produce volatile fatty acids (VFAs), such as acetic, propionic, and butyric acids, from lignocellulosic biomass. Typical 12 species of ruminal bacteria were detected in the culture broth cultivated by RMS using species-specific PCR assay. These species are known to be cellulolytic, hemicellulolytic, proteolytic, amylolytic, and lipolytic bacteria and methanogens. Therefore, RMS was characterized to be ruminal fermentation like the digestive system of roughage in the rumen of cattle. Using RMS, the ruminal bacteria were continuously cultivated for 120 days to produce VFAs. The yield of total VFAs from 20–50 μm rice straw was 167% higher than that from 300–1000 μm rice straw. However, the productivity was not increased by pulverization of the substrate. The yield and productivity of acetic acid from 20–50 μm rice straw were 0.147 g/g and 34.8 mg/L/h, respectively, and those of propionic acid were 0.104 g/g and 24.6 mg/L/h, respectively. The selectivity (P/A ratio) of acetic and propionic acid production from microcrystalline cellulose at pH 6.5 and 7.0 were 0.83±0.07 and 0.31±0.03, respectively. The ruminal bacteria could not continue to produce VFAs at pH 6.0. The yield and productivity of acetic acid from microcrystalline cellulose at pH 6.5 were 0.234 g/g and 51.7 mg/L/h, respectively, and those of propionic acid were 0.223 g/g and 49.3 mg/L/h, respectively. This study proposes that in order to increase the productivity of RMS, it is necessary to increase the ruminal bacterial biomass by recycling biomass (flocs) using a settling tank.
| Published in | International Journal of Microbiology and Biotechnology (Volume 6, Issue 1) |
| DOI | 10.11648/j.ijmb.20210601.11 |
| Page(s) | 1-8 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Rumen, Ruminal Bacteria, Volatile Fatty Acid, Acetic Acid, Propionic Acid, Lignocellulosic Biomass
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APA Style
Hitosi Agematu, Jun Watanabe, Takehiko Takahashi. (2021). Characterization of the Rumen-Mimetic Continuous Cultivation System for Volatile Fatty Acid Production from Lignocellulosic Biomass. International Journal of Microbiology and Biotechnology, 6(1), 1-8. https://doi.org/10.11648/j.ijmb.20210601.11
ACS Style
Hitosi Agematu; Jun Watanabe; Takehiko Takahashi. Characterization of the Rumen-Mimetic Continuous Cultivation System for Volatile Fatty Acid Production from Lignocellulosic Biomass. Int. J. Microbiol. Biotechnol. 2021, 6(1), 1-8. doi: 10.11648/j.ijmb.20210601.11
AMA Style
Hitosi Agematu, Jun Watanabe, Takehiko Takahashi. Characterization of the Rumen-Mimetic Continuous Cultivation System for Volatile Fatty Acid Production from Lignocellulosic Biomass. Int J Microbiol Biotechnol. 2021;6(1):1-8. doi: 10.11648/j.ijmb.20210601.11
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Download@article{10.11648/j.ijmb.20210601.11,
author = {Hitosi Agematu and Jun Watanabe and Takehiko Takahashi},
title = {Characterization of the Rumen-Mimetic Continuous Cultivation System for Volatile Fatty Acid Production from Lignocellulosic Biomass},
journal = {International Journal of Microbiology and Biotechnology},
volume = {6},
number = {1},
pages = {1-8},
doi = {10.11648/j.ijmb.20210601.11},
url = {https://doi.org/10.11648/j.ijmb.20210601.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20210601.11},
abstract = {The rumen-mimetic continuous cultivation system (RMS) was used to produce volatile fatty acids (VFAs), such as acetic, propionic, and butyric acids, from lignocellulosic biomass. Typical 12 species of ruminal bacteria were detected in the culture broth cultivated by RMS using species-specific PCR assay. These species are known to be cellulolytic, hemicellulolytic, proteolytic, amylolytic, and lipolytic bacteria and methanogens. Therefore, RMS was characterized to be ruminal fermentation like the digestive system of roughage in the rumen of cattle. Using RMS, the ruminal bacteria were continuously cultivated for 120 days to produce VFAs. The yield of total VFAs from 20–50 μm rice straw was 167% higher than that from 300–1000 μm rice straw. However, the productivity was not increased by pulverization of the substrate. The yield and productivity of acetic acid from 20–50 μm rice straw were 0.147 g/g and 34.8 mg/L/h, respectively, and those of propionic acid were 0.104 g/g and 24.6 mg/L/h, respectively. The selectivity (P/A ratio) of acetic and propionic acid production from microcrystalline cellulose at pH 6.5 and 7.0 were 0.83±0.07 and 0.31±0.03, respectively. The ruminal bacteria could not continue to produce VFAs at pH 6.0. The yield and productivity of acetic acid from microcrystalline cellulose at pH 6.5 were 0.234 g/g and 51.7 mg/L/h, respectively, and those of propionic acid were 0.223 g/g and 49.3 mg/L/h, respectively. This study proposes that in order to increase the productivity of RMS, it is necessary to increase the ruminal bacterial biomass by recycling biomass (flocs) using a settling tank.},
year = {2021}
}
TY - JOUR T1 - Characterization of the Rumen-Mimetic Continuous Cultivation System for Volatile Fatty Acid Production from Lignocellulosic Biomass AU - Hitosi Agematu AU - Jun Watanabe AU - Takehiko Takahashi Y1 - 2021/02/23 PY - 2021 N1 - https://doi.org/10.11648/j.ijmb.20210601.11 DO - 10.11648/j.ijmb.20210601.11 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 1 EP - 8 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20210601.11 AB - The rumen-mimetic continuous cultivation system (RMS) was used to produce volatile fatty acids (VFAs), such as acetic, propionic, and butyric acids, from lignocellulosic biomass. Typical 12 species of ruminal bacteria were detected in the culture broth cultivated by RMS using species-specific PCR assay. These species are known to be cellulolytic, hemicellulolytic, proteolytic, amylolytic, and lipolytic bacteria and methanogens. Therefore, RMS was characterized to be ruminal fermentation like the digestive system of roughage in the rumen of cattle. Using RMS, the ruminal bacteria were continuously cultivated for 120 days to produce VFAs. The yield of total VFAs from 20–50 μm rice straw was 167% higher than that from 300–1000 μm rice straw. However, the productivity was not increased by pulverization of the substrate. The yield and productivity of acetic acid from 20–50 μm rice straw were 0.147 g/g and 34.8 mg/L/h, respectively, and those of propionic acid were 0.104 g/g and 24.6 mg/L/h, respectively. The selectivity (P/A ratio) of acetic and propionic acid production from microcrystalline cellulose at pH 6.5 and 7.0 were 0.83±0.07 and 0.31±0.03, respectively. The ruminal bacteria could not continue to produce VFAs at pH 6.0. The yield and productivity of acetic acid from microcrystalline cellulose at pH 6.5 were 0.234 g/g and 51.7 mg/L/h, respectively, and those of propionic acid were 0.223 g/g and 49.3 mg/L/h, respectively. This study proposes that in order to increase the productivity of RMS, it is necessary to increase the ruminal bacterial biomass by recycling biomass (flocs) using a settling tank. VL - 6 IS - 1 ER -
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