Marine microorganisms are the important resources for natural drug discovery. However, most of genes are usually in silent and could not express under the condition of traditional culture, which limits the discovery of lead compounds. In the present research, marine fungus Schizophyllum sp. YS-08 was selected as the research object, which was from Yellow Sea of China, to activate its silent genes by changing the medium of nutrients and environmental conditions. The crude extracts from metabolites of marine fungi were analyzed by HPLC, and their antioxidant activity and acetylcholinesterase (AChE) inhibitory activity were studied by DPPH radical-scavenging and Ellman's method, respectively. The results indicated that metabolic pathway of fungus was regulated effectively in different culture conditions, especially in the normal sea water medium with peptone. The antioxidant activity and AChE inhibitory activity increased significantly in comparison with wild strain and increase of the quantity of chemical constituents were even more. It is also observed that the strains showed relatively slow growth in the high salinity situation, while this adversely environmental condition could promote and produce more active metabolites. Furthermore, 6 mutant strains were obtained under salt stress and were identified. Interestingly, we found that all mutant strains had potency toward antioxidant and AChE inhibitory activity. Among them, YS-08-2 was the most potent, scavenging more than 56.02% towards DPPH free radicals, and YS-08-1, YS-08-4 and YS-08-5 exhibited more than 40% inhibition against AChE. HPLC analysis of extracts showed the metabolites of most fungi were more abundant after regulation of culture conditions.
| Published in | International Journal of Microbiology and Biotechnology (Volume 6, Issue 3) |
| DOI | 10.11648/j.ijmb.20210603.13 |
| Page(s) | 78-85 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Symbiotic Fungi, OSMAC Strategy, Secondary Metabolites, Antioxidant Activity, AChE Inhibitory Activity
| [1] | Li G., Lou H. X. (2018). Strategies to diversify natural products for drug discovery. Med Res Rev, 38, 1255-1294. |
| [2] | Blunt J. W., Carroll A. R., Copp B. R., et al. (2018). Marine natural products. Nat Prod Rep, 35, 8-53. |
| [3] | Carroll A. R., Copp B. R., Rohan A., et al. (2019). Marine natural products. Nat Prod Rep, 36, 122-173. |
| [4] | Amend A., Burgaud G., Cunliffe M., et al. (2019). Fungi in the marine environment: open questions and unsolved problems. mBio, 10, e01189-18. |
| [5] | Paranagama P. A., Wijeratne E. M. K., Gunatilaka A. L. (2007). Μncovering biosynthetic potential of plant-associated fungi: effect of culture conditions on metabolite production by paraphaeosphaeria quadriseptata and chaetomium chiversii. J Nat Prod, 70, 1939-1945. |
| [6] | Pu X., Qu X. X., Chen F., et al. (2013). Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production. Appl Microbiol Biot, 97, 9365-9375. |
| [7] | Wang W. X., Kusari S., Spiteller M. (2016). Unraveling the chemical interactions of fungal endophytes for exploitation as microbial factories. Fungal Appl Sustain Environ Biotech, 353-370. |
| [8] | Yu P. W., Cho T. Y., Lioμ R. F., et al. (2017). Identification of the orsellinic acid synthase PKS63787 for the biosynthesis of antroquinonols in antrodia cinnamomea. Appl Microbiol Biot, 101, 4701-4711. |
| [9] | Stroe M. C., Netzker T., Scherlach K., et al. (2020). Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin. eLife, 9, e52541. |
| [10] | Yang Z. J., He J. Q., Wei X., et al. (2020). Exploration and genome mining of natural products from marine Streptomyces. Appl Microbiol Biot, 104, 67-76. |
| [11] | Bauman K. D., Li J., Murata K., et al. (2019). Refactoring the cryptic streptophenazine biosynthetic gene cluster unites phenazine, polyketide, and nonribosomal peptide biochemistry. Cell Chem Biol, 26, 724-736. |
| [12] | Kozlovsky A. G., Zhelifonova V. P., Antipova T. V., et al. (2010). The influence of medium compositiun on alkaloid biosynthesis by penicillium citrinum. Appl Biochem Micro, 46, 525-529. |
| [13] | Romano S., Jackson S. A., Patry S., et al. (2018). Extending the "One Strain Many Compounds" (OSMAC) principle to marine microorganisms. Mar Drugs, 16, 244. |
| [14] | Pan R., Bai X. L., Chen J. W., et al. (2019). Exploring structural diversity of microbe secondary metabolites using OSMAC strategy: a literature review. Front Microbiol, 10, 294. |
| [15] | Reen F. J., Romano S., Dobson A. D. W., et al. (2015). The sound of silence: activating silent biosynthetic gene clusters in marine microorganisms. Mar Drugs, 13, 4754-4783. |
| [16] | Chai Y. J., Cui C. B., Li C. W., et al. (2012). Activation of the dormant secondary metabolite production by introducing gentamicin-resistance in a marine-derived penicillium purpurogenum G59. Mar Drugs, 10, 559-582. |
| [17] | Lin Z. J., Zhu T. J., Wei H. J., et al. (2019). Spicochalasin A and new aspochalasins from the marine-derived fungus spicaria elegans. Eur J Org Chem, 18, 3045-3051. |
| [18] | Abdelmohsen U. R., Cheng C., Viegelmann C., et al. (2014). Dereplication strategies for targeted isolation of new antitrypanosomal actinosporins A and B from a marine sponge associated-actinokineospora sp EG49. Mar Drugs, 12, 1220-1244. |
| [19] | Hewage R. T., Aree T., Mahidol C., et al. (2014). One strain-many compounds (OSMAC) method for production of polyketides, azaphilones, and an isochromanone using the endophytic fungus Dothideomycete sp. Phytochemistry, 108, 87-94. |
| [20] | Zang Y., Gong Y. H., Gong J. J., et al. (2020). Fungal Polyketides with three distinctive ring skeletons from the fungus penicillium canescens uncovered by OSMAC and molecular networking strategies. J Org Chem, 85, 4973-4980. |
| [21] | Almeida E. L., Kaur N., Jennings L. K., et al. (2019). Genome mining coupled with OSMAC-based cultivation reveal differential production of surugamide A by the marine sponge isolate streptomyces sp. SM17 when compared to its terrestrial relative S. albidoflavus J1074. Microorganisms, 7, 394. |
| [22] | Zhao D. L., Han X. B., Wang D., et al. (2019). Bioactive 3-decalinoyltetramic acids derivatives from a marine-derived strain of the fungus fusarium equiseti D39. Front Microbiol 10, 1285. |
| [23] | Li W., Ding L. J., Wang N., et al. (2019). Isolation and characterization of two new metabolites from the sponge-derived fungus Aspergillus sp. LS34 by OSMAC approach. Mar Drugs, 17, 283. |
| [24] | Zhang Z. Z., He X. Q., Che Q., et al. (2018). Sorbicillasins A-B and scirpyrone K from a deep-sea-derived fungus, phialocephala sp FL30r. Mar Drugs, 16, 245. |
| [25] | Wang H. N., Yang H., Gu Y. L., et al. (2019). Study on isolation, indentification and bioactivity of secondary metabolites from oysters symbiotic bacteria. J Liaocheng University, 32, 106-110. |
| [26] | Saitou N., Nei M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol, 4, 406-425. |
| [27] | Foti M. C. (2015). The use and abuse of the DPPH• radical. J Agr Food Chem, 63, 8765. |
| [28] | Zaki A. G., El-Shatoury E. H., Ahmed A. S., et al. (2019). Production and enhancement of the acetylcholinesterase inhibitor, huperzine A, from an endophytic Alternariabrassicae AGF041. Appl Microbiol Biot, 103, 5867-5878. |
| [29] | Wang Y., Zheng J. K., Liu P. P., et al. (2011). Three new compounds from aspergillμs terreus PT06-2 grown in a high salt medium. Mar Drugs, 9, 1368-1378. |
| [30] | Huang J. J., Lu C. H., Qian X. M., et al. (2011). Effect of salinity on the growth, biological activity and secondary metabolites of some marine fungi. Acta Oceanol Sin, 30, 118-123. |
| [31] | Marmann A., Aly A. H., Lin W. H., et al. (2014). Co-cultivation-a powerful emerging tool for enhancing the chemical diversity of microorganisms. Mar Drugs, 12, 1043-1065. |
| [32] | Bode H. B., Bethe B., Hofs R., et al. (2002). Big effects from small changes: possible ways to explore nature's chemical diversity. Chembiochem, 3, 619-627. |
APA Style
Huannan Wang, Maocai Yan, Mengdi Liang, Xiujian Wei, Zhao Shang, et al. (2021). Regulation and Activity Evaluation of Secondary Metabolism of an Oyster Symbiotic Fungus Schizophyllum sp. YS-08. International Journal of Microbiology and Biotechnology, 6(3), 78-85. https://doi.org/10.11648/j.ijmb.20210603.13
ACS Style
Huannan Wang; Maocai Yan; Mengdi Liang; Xiujian Wei; Zhao Shang, et al. Regulation and Activity Evaluation of Secondary Metabolism of an Oyster Symbiotic Fungus Schizophyllum sp. YS-08. Int. J. Microbiol. Biotechnol. 2021, 6(3), 78-85. doi: 10.11648/j.ijmb.20210603.13
AMA Style
Huannan Wang, Maocai Yan, Mengdi Liang, Xiujian Wei, Zhao Shang, et al. Regulation and Activity Evaluation of Secondary Metabolism of an Oyster Symbiotic Fungus Schizophyllum sp. YS-08. Int J Microbiol Biotechnol. 2021;6(3):78-85. doi: 10.11648/j.ijmb.20210603.13
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Download@article{10.11648/j.ijmb.20210603.13,
author = {Huannan Wang and Maocai Yan and Mengdi Liang and Xiujian Wei and Zhao Shang and Zhen Zhang},
title = {Regulation and Activity Evaluation of Secondary Metabolism of an Oyster Symbiotic Fungus Schizophyllum sp. YS-08},
journal = {International Journal of Microbiology and Biotechnology},
volume = {6},
number = {3},
pages = {78-85},
doi = {10.11648/j.ijmb.20210603.13},
url = {https://doi.org/10.11648/j.ijmb.20210603.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20210603.13},
abstract = {Marine microorganisms are the important resources for natural drug discovery. However, most of genes are usually in silent and could not express under the condition of traditional culture, which limits the discovery of lead compounds. In the present research, marine fungus Schizophyllum sp. YS-08 was selected as the research object, which was from Yellow Sea of China, to activate its silent genes by changing the medium of nutrients and environmental conditions. The crude extracts from metabolites of marine fungi were analyzed by HPLC, and their antioxidant activity and acetylcholinesterase (AChE) inhibitory activity were studied by DPPH radical-scavenging and Ellman's method, respectively. The results indicated that metabolic pathway of fungus was regulated effectively in different culture conditions, especially in the normal sea water medium with peptone. The antioxidant activity and AChE inhibitory activity increased significantly in comparison with wild strain and increase of the quantity of chemical constituents were even more. It is also observed that the strains showed relatively slow growth in the high salinity situation, while this adversely environmental condition could promote and produce more active metabolites. Furthermore, 6 mutant strains were obtained under salt stress and were identified. Interestingly, we found that all mutant strains had potency toward antioxidant and AChE inhibitory activity. Among them, YS-08-2 was the most potent, scavenging more than 56.02% towards DPPH free radicals, and YS-08-1, YS-08-4 and YS-08-5 exhibited more than 40% inhibition against AChE. HPLC analysis of extracts showed the metabolites of most fungi were more abundant after regulation of culture conditions.},
year = {2021}
}
TY - JOUR T1 - Regulation and Activity Evaluation of Secondary Metabolism of an Oyster Symbiotic Fungus Schizophyllum sp. YS-08 AU - Huannan Wang AU - Maocai Yan AU - Mengdi Liang AU - Xiujian Wei AU - Zhao Shang AU - Zhen Zhang Y1 - 2021/08/09 PY - 2021 N1 - https://doi.org/10.11648/j.ijmb.20210603.13 DO - 10.11648/j.ijmb.20210603.13 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 78 EP - 85 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20210603.13 AB - Marine microorganisms are the important resources for natural drug discovery. However, most of genes are usually in silent and could not express under the condition of traditional culture, which limits the discovery of lead compounds. In the present research, marine fungus Schizophyllum sp. YS-08 was selected as the research object, which was from Yellow Sea of China, to activate its silent genes by changing the medium of nutrients and environmental conditions. The crude extracts from metabolites of marine fungi were analyzed by HPLC, and their antioxidant activity and acetylcholinesterase (AChE) inhibitory activity were studied by DPPH radical-scavenging and Ellman's method, respectively. The results indicated that metabolic pathway of fungus was regulated effectively in different culture conditions, especially in the normal sea water medium with peptone. The antioxidant activity and AChE inhibitory activity increased significantly in comparison with wild strain and increase of the quantity of chemical constituents were even more. It is also observed that the strains showed relatively slow growth in the high salinity situation, while this adversely environmental condition could promote and produce more active metabolites. Furthermore, 6 mutant strains were obtained under salt stress and were identified. Interestingly, we found that all mutant strains had potency toward antioxidant and AChE inhibitory activity. Among them, YS-08-2 was the most potent, scavenging more than 56.02% towards DPPH free radicals, and YS-08-1, YS-08-4 and YS-08-5 exhibited more than 40% inhibition against AChE. HPLC analysis of extracts showed the metabolites of most fungi were more abundant after regulation of culture conditions. VL - 6 IS - 3 ER -
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