In <i>Vitro </i>Evaluation of Putative Probiotic Candidates Isolated from Various Origins

Nilofar Yunus Bhatti; Arati Savji Chavda; Bharatkumar Rajiv Manuel Vyas

doi:doi:10.11648/j.ijmb.20230801.11

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In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins

Nilofar Yunus Bhatti, Arati Savji Chavda, Bharatkumar Rajiv Manuel Vyas

Published in International Journal of Microbiology and Biotechnology (Volume 8, Issue 1)

Received: 12 August 2022 Accepted: 7 September 2022 Published: 13 January 2023

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Abstract

Present study describes isolation of potential probiotic lactic acid bacteria from chicken crop, human feces, buttermilk and chilly. The isolated Lactobacillus strains survive, tolerate and grow in MRS medium spiked with bile, salt and having acidic pH. The Lactobacillus isolates possess several probiotic properties, viz. (i) ability to bind gastrointestinal mucosa, up to ≥80% cells adhered mucin, (ii) 50% cells retained viability during oro-gastro-intestinal transit, (iii) all the isolates exhibited broad anti-microbial spectrum against food spoilage and gastro-intestinal pathogens, Limosilactobacillus fermentum SBM showed maximum inhibition, (iv) ability to produce enzymatic activities like L-asparaginase, β-galactosidase and bile salt hydrolase (BSH) activities, Limosilactobacillus fermentum SBM showed maximum L-asparaginase activity (2.567 U/ml), and Lactiplantibacillus pentosus GCHI showed maximum β-galactosidase activity (296±0.1 Miller’s Unit), (v) Lactiplantibacillus pentosus GCHI aggregated up to ≥92% after 24 h, and (vi) the Lactobacillus isolates were susceptible towards nucleic acid synthesis inhibitors and cell wall synthesis inhibitor antibiotics. These Lactobacillus strains do not possess haemolytic, mucin degrading and DNase activities indicating their safety. Further characterization of these strains indicated potential probiotic properties and their suitability in food formulations as probiotics. The study presents an interesting illustration of mining of potential probiotic strains from nature exhibiting health benefits for human being and animals.

Published in	International Journal of Microbiology and Biotechnology (Volume 8, Issue 1)
DOI	10.11648/j.ijmb.20230801.11
Page(s)	1-9
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Anti-Microbial Activity, BSH, Lactobacillus Strains, L-asparaginase, Probiotics, Safety Evaluation

References

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Nilofar Yunus Bhatti, Arati Savji Chavda, Bharatkumar Rajiv Manuel Vyas. (2023). In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins. International Journal of Microbiology and Biotechnology, 8(1), 1-9. https://doi.org/10.11648/j.ijmb.20230801.11

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Nilofar Yunus Bhatti; Arati Savji Chavda; Bharatkumar Rajiv Manuel Vyas. In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins. Int. J. Microbiol. Biotechnol. 2023, 8(1), 1-9. doi: 10.11648/j.ijmb.20230801.11

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AMA Style

Nilofar Yunus Bhatti, Arati Savji Chavda, Bharatkumar Rajiv Manuel Vyas. In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins. Int J Microbiol Biotechnol. 2023;8(1):1-9. doi: 10.11648/j.ijmb.20230801.11

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@article{10.11648/j.ijmb.20230801.11,
  author = {Nilofar Yunus Bhatti and Arati Savji Chavda and Bharatkumar Rajiv Manuel Vyas},
  title = {In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {8},
  number = {1},
  pages = {1-9},
  doi = {10.11648/j.ijmb.20230801.11},
  url = {https://doi.org/10.11648/j.ijmb.20230801.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20230801.11},
  abstract = {Present study describes isolation of potential probiotic lactic acid bacteria from chicken crop, human feces, buttermilk and chilly. The isolated Lactobacillus strains survive, tolerate and grow in MRS medium spiked with bile, salt and having acidic pH. The Lactobacillus isolates possess several probiotic properties, viz. (i) ability to bind gastrointestinal mucosa, up to ≥80% cells adhered mucin, (ii) 50% cells retained viability during oro-gastro-intestinal transit, (iii) all the isolates exhibited broad anti-microbial spectrum against food spoilage and gastro-intestinal pathogens, Limosilactobacillus fermentum SBM showed maximum inhibition, (iv) ability to produce enzymatic activities like L-asparaginase, β-galactosidase and bile salt hydrolase (BSH) activities, Limosilactobacillus fermentum SBM showed maximum L-asparaginase activity (2.567 U/ml), and Lactiplantibacillus pentosus GCHI showed maximum β-galactosidase activity (296±0.1 Miller’s Unit), (v) Lactiplantibacillus pentosus GCHI aggregated up to ≥92% after 24 h, and (vi) the Lactobacillus isolates were susceptible towards nucleic acid synthesis inhibitors and cell wall synthesis inhibitor antibiotics. These Lactobacillus strains do not possess haemolytic, mucin degrading and DNase activities indicating their safety. Further characterization of these strains indicated potential probiotic properties and their suitability in food formulations as probiotics. The study presents an interesting illustration of mining of potential probiotic strains from nature exhibiting health benefits for human being and animals.},
 year = {2023}
}

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TY  - JOUR
T1  - In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins
AU  - Nilofar Yunus Bhatti
AU  - Arati Savji Chavda
AU  - Bharatkumar Rajiv Manuel Vyas
Y1  - 2023/01/13
PY  - 2023
N1  - https://doi.org/10.11648/j.ijmb.20230801.11
DO  - 10.11648/j.ijmb.20230801.11
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 1
EP  - 9
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20230801.11
AB  - Present study describes isolation of potential probiotic lactic acid bacteria from chicken crop, human feces, buttermilk and chilly. The isolated Lactobacillus strains survive, tolerate and grow in MRS medium spiked with bile, salt and having acidic pH. The Lactobacillus isolates possess several probiotic properties, viz. (i) ability to bind gastrointestinal mucosa, up to ≥80% cells adhered mucin, (ii) 50% cells retained viability during oro-gastro-intestinal transit, (iii) all the isolates exhibited broad anti-microbial spectrum against food spoilage and gastro-intestinal pathogens, Limosilactobacillus fermentum SBM showed maximum inhibition, (iv) ability to produce enzymatic activities like L-asparaginase, β-galactosidase and bile salt hydrolase (BSH) activities, Limosilactobacillus fermentum SBM showed maximum L-asparaginase activity (2.567 U/ml), and Lactiplantibacillus pentosus GCHI showed maximum β-galactosidase activity (296±0.1 Miller’s Unit), (v) Lactiplantibacillus pentosus GCHI aggregated up to ≥92% after 24 h, and (vi) the Lactobacillus isolates were susceptible towards nucleic acid synthesis inhibitors and cell wall synthesis inhibitor antibiotics. These Lactobacillus strains do not possess haemolytic, mucin degrading and DNase activities indicating their safety. Further characterization of these strains indicated potential probiotic properties and their suitability in food formulations as probiotics. The study presents an interesting illustration of mining of potential probiotic strains from nature exhibiting health benefits for human being and animals.
VL  - 8
IS  - 1
ER  -

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Author Information

Nilofar Yunus Bhatti

Department of Biosciences, Saurashtra University, Rajkot, India
Arati Savji Chavda

Department of Biosciences, Saurashtra University, Rajkot, India
Bharatkumar Rajiv Manuel Vyas

Department of Biosciences, Saurashtra University, Rajkot, India

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Nilofar Yunus Bhatti, Arati Savji Chavda, Bharatkumar Rajiv Manuel Vyas. (2023). In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins. International Journal of Microbiology and Biotechnology, 8(1), 1-9. https://doi.org/10.11648/j.ijmb.20230801.11

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ACS Style

Nilofar Yunus Bhatti; Arati Savji Chavda; Bharatkumar Rajiv Manuel Vyas. In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins. Int. J. Microbiol. Biotechnol. 2023, 8(1), 1-9. doi: 10.11648/j.ijmb.20230801.11

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AMA Style

Nilofar Yunus Bhatti, Arati Savji Chavda, Bharatkumar Rajiv Manuel Vyas. In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins. Int J Microbiol Biotechnol. 2023;8(1):1-9. doi: 10.11648/j.ijmb.20230801.11

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@article{10.11648/j.ijmb.20230801.11,
  author = {Nilofar Yunus Bhatti and Arati Savji Chavda and Bharatkumar Rajiv Manuel Vyas},
  title = {In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {8},
  number = {1},
  pages = {1-9},
  doi = {10.11648/j.ijmb.20230801.11},
  url = {https://doi.org/10.11648/j.ijmb.20230801.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20230801.11},
  abstract = {Present study describes isolation of potential probiotic lactic acid bacteria from chicken crop, human feces, buttermilk and chilly. The isolated Lactobacillus strains survive, tolerate and grow in MRS medium spiked with bile, salt and having acidic pH. The Lactobacillus isolates possess several probiotic properties, viz. (i) ability to bind gastrointestinal mucosa, up to ≥80% cells adhered mucin, (ii) 50% cells retained viability during oro-gastro-intestinal transit, (iii) all the isolates exhibited broad anti-microbial spectrum against food spoilage and gastro-intestinal pathogens, Limosilactobacillus fermentum SBM showed maximum inhibition, (iv) ability to produce enzymatic activities like L-asparaginase, β-galactosidase and bile salt hydrolase (BSH) activities, Limosilactobacillus fermentum SBM showed maximum L-asparaginase activity (2.567 U/ml), and Lactiplantibacillus pentosus GCHI showed maximum β-galactosidase activity (296±0.1 Miller’s Unit), (v) Lactiplantibacillus pentosus GCHI aggregated up to ≥92% after 24 h, and (vi) the Lactobacillus isolates were susceptible towards nucleic acid synthesis inhibitors and cell wall synthesis inhibitor antibiotics. These Lactobacillus strains do not possess haemolytic, mucin degrading and DNase activities indicating their safety. Further characterization of these strains indicated potential probiotic properties and their suitability in food formulations as probiotics. The study presents an interesting illustration of mining of potential probiotic strains from nature exhibiting health benefits for human being and animals.},
 year = {2023}
}

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TY  - JOUR
T1  - In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins
AU  - Nilofar Yunus Bhatti
AU  - Arati Savji Chavda
AU  - Bharatkumar Rajiv Manuel Vyas
Y1  - 2023/01/13
PY  - 2023
N1  - https://doi.org/10.11648/j.ijmb.20230801.11
DO  - 10.11648/j.ijmb.20230801.11
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 1
EP  - 9
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20230801.11
AB  - Present study describes isolation of potential probiotic lactic acid bacteria from chicken crop, human feces, buttermilk and chilly. The isolated Lactobacillus strains survive, tolerate and grow in MRS medium spiked with bile, salt and having acidic pH. The Lactobacillus isolates possess several probiotic properties, viz. (i) ability to bind gastrointestinal mucosa, up to ≥80% cells adhered mucin, (ii) 50% cells retained viability during oro-gastro-intestinal transit, (iii) all the isolates exhibited broad anti-microbial spectrum against food spoilage and gastro-intestinal pathogens, Limosilactobacillus fermentum SBM showed maximum inhibition, (iv) ability to produce enzymatic activities like L-asparaginase, β-galactosidase and bile salt hydrolase (BSH) activities, Limosilactobacillus fermentum SBM showed maximum L-asparaginase activity (2.567 U/ml), and Lactiplantibacillus pentosus GCHI showed maximum β-galactosidase activity (296±0.1 Miller’s Unit), (v) Lactiplantibacillus pentosus GCHI aggregated up to ≥92% after 24 h, and (vi) the Lactobacillus isolates were susceptible towards nucleic acid synthesis inhibitors and cell wall synthesis inhibitor antibiotics. These Lactobacillus strains do not possess haemolytic, mucin degrading and DNase activities indicating their safety. Further characterization of these strains indicated potential probiotic properties and their suitability in food formulations as probiotics. The study presents an interesting illustration of mining of potential probiotic strains from nature exhibiting health benefits for human being and animals.
VL  - 8
IS  - 1
ER  -

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