Marek's disease virus (MDV) is a highly cell-associated oncogenic alphaherpesvirus that causes chicken T-cell lymphoma. MD is currently controlled by vaccination; however, MDV strains tend to develop increased virulence. Distinct diversity and point mutations are present in the Meq proteins, the major oncoproteins of MDV, suggesting that changes in protein function induced by amino acid substitutions might affect MDV virulence. This study sampled 30 commercial broiler flocks from different Provinces (10 spleens from each flock) at slaughter. Gallid alphaherpesvirus 2 was identified in PCR (using gb primer) of spleen samples of 11 flocks (36.67%). Two provinces, Azerbaijan Gharbi (44%) and Golestan (25%), recorded the highest and lowest infection rates. The oncogene Meq of some positive samples was amplified by PCR and sequenced. MDV strains detected in this study could be put in three branches, with molecular features consistent with virulent and very virulent previously identified MDV. UT-PCR9303 was located with an Iraqi isolate. UT-PCR-9231 had high homology with an Iranian MDV sequence detected from a layer farm with MD. UT-PCR-9380 was located with vv MDV from Japan and Colombia. Therefore, the relatively high rate of Meq in the unvaccinated broiler farms constitutes support for vaccination. These findings provide the basis for molecular surveillance and further study of MDV mutants and strategies for managing MD in Iran.
| Published in | International Journal of Microbiology and Biotechnology (Volume 8, Issue 3) |
| DOI | 10.11648/j.ijmb.20230803.11 |
| Page(s) | 48-53 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Marek Disease, Iran, Broiler, Phylogenetic Analysis, Meq
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APA Style
Rima Morshed, Hossein Hosseini, Naser Sadri, Zahra Ziafati Kafi, Soroush Sarmadi, et al. (2023). Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study. International Journal of Microbiology and Biotechnology, 8(3), 48-53. https://doi.org/10.11648/j.ijmb.20230803.11
ACS Style
Rima Morshed; Hossein Hosseini; Naser Sadri; Zahra Ziafati Kafi; Soroush Sarmadi, et al. Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study. Int. J. Microbiol. Biotechnol. 2023, 8(3), 48-53. doi: 10.11648/j.ijmb.20230803.11
AMA Style
Rima Morshed, Hossein Hosseini, Naser Sadri, Zahra Ziafati Kafi, Soroush Sarmadi, et al. Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study. Int J Microbiol Biotechnol. 2023;8(3):48-53. doi: 10.11648/j.ijmb.20230803.11
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Download@article{10.11648/j.ijmb.20230803.11,
author = {Rima Morshed and Hossein Hosseini and Naser Sadri and Zahra Ziafati Kafi and Soroush Sarmadi and Nazanin Sarvian and Seyed Hesamodin Emadi Chashmi and Farhad Arab Baghi and Arash Ghalyanchilangeroudi},
title = {Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study},
journal = {International Journal of Microbiology and Biotechnology},
volume = {8},
number = {3},
pages = {48-53},
doi = {10.11648/j.ijmb.20230803.11},
url = {https://doi.org/10.11648/j.ijmb.20230803.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20230803.11},
abstract = {Marek's disease virus (MDV) is a highly cell-associated oncogenic alphaherpesvirus that causes chicken T-cell lymphoma. MD is currently controlled by vaccination; however, MDV strains tend to develop increased virulence. Distinct diversity and point mutations are present in the Meq proteins, the major oncoproteins of MDV, suggesting that changes in protein function induced by amino acid substitutions might affect MDV virulence. This study sampled 30 commercial broiler flocks from different Provinces (10 spleens from each flock) at slaughter. Gallid alphaherpesvirus 2 was identified in PCR (using gb primer) of spleen samples of 11 flocks (36.67%). Two provinces, Azerbaijan Gharbi (44%) and Golestan (25%), recorded the highest and lowest infection rates. The oncogene Meq of some positive samples was amplified by PCR and sequenced. MDV strains detected in this study could be put in three branches, with molecular features consistent with virulent and very virulent previously identified MDV. UT-PCR9303 was located with an Iraqi isolate. UT-PCR-9231 had high homology with an Iranian MDV sequence detected from a layer farm with MD. UT-PCR-9380 was located with vv MDV from Japan and Colombia. Therefore, the relatively high rate of Meq in the unvaccinated broiler farms constitutes support for vaccination. These findings provide the basis for molecular surveillance and further study of MDV mutants and strategies for managing MD in Iran.},
year = {2023}
}
TY - JOUR T1 - Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study AU - Rima Morshed AU - Hossein Hosseini AU - Naser Sadri AU - Zahra Ziafati Kafi AU - Soroush Sarmadi AU - Nazanin Sarvian AU - Seyed Hesamodin Emadi Chashmi AU - Farhad Arab Baghi AU - Arash Ghalyanchilangeroudi Y1 - 2023/07/31 PY - 2023 N1 - https://doi.org/10.11648/j.ijmb.20230803.11 DO - 10.11648/j.ijmb.20230803.11 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 48 EP - 53 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20230803.11 AB - Marek's disease virus (MDV) is a highly cell-associated oncogenic alphaherpesvirus that causes chicken T-cell lymphoma. MD is currently controlled by vaccination; however, MDV strains tend to develop increased virulence. Distinct diversity and point mutations are present in the Meq proteins, the major oncoproteins of MDV, suggesting that changes in protein function induced by amino acid substitutions might affect MDV virulence. This study sampled 30 commercial broiler flocks from different Provinces (10 spleens from each flock) at slaughter. Gallid alphaherpesvirus 2 was identified in PCR (using gb primer) of spleen samples of 11 flocks (36.67%). Two provinces, Azerbaijan Gharbi (44%) and Golestan (25%), recorded the highest and lowest infection rates. The oncogene Meq of some positive samples was amplified by PCR and sequenced. MDV strains detected in this study could be put in three branches, with molecular features consistent with virulent and very virulent previously identified MDV. UT-PCR9303 was located with an Iraqi isolate. UT-PCR-9231 had high homology with an Iranian MDV sequence detected from a layer farm with MD. UT-PCR-9380 was located with vv MDV from Japan and Colombia. Therefore, the relatively high rate of Meq in the unvaccinated broiler farms constitutes support for vaccination. These findings provide the basis for molecular surveillance and further study of MDV mutants and strategies for managing MD in Iran. VL - 8 IS - 3 ER -
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