Bacterial virulence is a key factor determining the outcome of each bacterial infection, and virulent bacteria are often associated with high-risk infections. Thus, this study aimed to screen for virulence genes and evaluate the in vitro biofilm formation capacity of multidrug-resistant Escherichia coli isolated in Dakar. For the 16 virulence genes identified by standard polymerase chain reaction (PCR), all 78 ExPEC isolates carried at least four virulence genes. The prevalence of virulence genes was as follows: adhesin genes fimH (98.7%), mrkD (98.7%), papC (46.2%), afaC (9%), and sfa/focDE (1.3%); iron acquisition system genes entB (98.7%), fepA (98.7%), ybtS (93.6%), fyuA (91%), iucA (91%), iucB (91%), iutA (34.6), iroB (6.4%), iroN (6.4%), and toxin genes hlyA (10.3%) and cnf (1 & 2) (10.3%). Seventy-five of the 78 isolates (96.2%) carried at least two adhesin genes and two iron capture system genes. Evaluation of the biofilm formation capacity revealed that all (29/29) hospital-acquired isolates were biofilm producers with (6/29; 20.7%) strong biofilm producers, (15/29; 51.7%) moderate biofilm producers and (8/29; 27.6%) weak biofilm producers. Hospital-acquired isolates carrying papC had a greater biofilm formation capacity than those lacking papC (p < 0.001). The deepening of this type of study on bacterial virulence and hospital bacterial biofilms could lead to improvements in infection investigation, prevention, and therapeutic protocols.
| Published in | International Journal of Microbiology and Biotechnology (Volume 8, Issue 4) |
| DOI | 10.11648/j.ijmb.20230804.11 |
| Page(s) | 69-81 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Virulence Genes, Virulence Factors, Extraintestinal Pathogenic Escherichia coli, Biofilms, Biofilm-Associated Infections
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APA Style
Komla Mawunyo Dossouvi, Bissoume Sambe Ba, Gora Lo, Issa Ndiaye, Awa Ba-Diallo, et al. (2023). High Prevalence of Virulence Genes and in Vitro Biofilm Production in Clinical Multidrug-Resistant Escherichia coli in Dakar Senegal. International Journal of Microbiology and Biotechnology, 8(4), 69-81. https://doi.org/10.11648/j.ijmb.20230804.11
ACS Style
Komla Mawunyo Dossouvi; Bissoume Sambe Ba; Gora Lo; Issa Ndiaye; Awa Ba-Diallo, et al. High Prevalence of Virulence Genes and in Vitro Biofilm Production in Clinical Multidrug-Resistant Escherichia coli in Dakar Senegal. Int. J. Microbiol. Biotechnol. 2023, 8(4), 69-81. doi: 10.11648/j.ijmb.20230804.11
AMA Style
Komla Mawunyo Dossouvi, Bissoume Sambe Ba, Gora Lo, Issa Ndiaye, Awa Ba-Diallo, et al. High Prevalence of Virulence Genes and in Vitro Biofilm Production in Clinical Multidrug-Resistant Escherichia coli in Dakar Senegal. Int J Microbiol Biotechnol. 2023;8(4):69-81. doi: 10.11648/j.ijmb.20230804.11
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author = {Komla Mawunyo Dossouvi and Bissoume Sambe Ba and Gora Lo and Issa Ndiaye and Awa Ba-Diallo and Ousmane Sow and Assane Dieng and Fatimata Talla and Abdoulaye Cissé and Serigne Mbaye Lo Ndiaye and Alioune Tine and Farba Karam and Habsa Diagne-Samb and Safietou Ngom-Cisse and Halimatou Diop-Ndiaye and Issaka Maman and Coumba Toure-Kane and Aïssatou Gaye-Diallo and Sika Dossim and Souleymane Mboup and Cheikh Saad Bouh Boye and Abdoulaye Seck and Makhtar Camara},
title = {High Prevalence of Virulence Genes and in Vitro Biofilm Production in Clinical Multidrug-Resistant Escherichia coli in Dakar Senegal},
journal = {International Journal of Microbiology and Biotechnology},
volume = {8},
number = {4},
pages = {69-81},
doi = {10.11648/j.ijmb.20230804.11},
url = {https://doi.org/10.11648/j.ijmb.20230804.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20230804.11},
abstract = {Bacterial virulence is a key factor determining the outcome of each bacterial infection, and virulent bacteria are often associated with high-risk infections. Thus, this study aimed to screen for virulence genes and evaluate the in vitro biofilm formation capacity of multidrug-resistant Escherichia coli isolated in Dakar. For the 16 virulence genes identified by standard polymerase chain reaction (PCR), all 78 ExPEC isolates carried at least four virulence genes. The prevalence of virulence genes was as follows: adhesin genes fimH (98.7%), mrkD (98.7%), papC (46.2%), afaC (9%), and sfa/focDE (1.3%); iron acquisition system genes entB (98.7%), fepA (98.7%), ybtS (93.6%), fyuA (91%), iucA (91%), iucB (91%), iutA (34.6), iroB (6.4%), iroN (6.4%), and toxin genes hlyA (10.3%) and cnf (1 & 2) (10.3%). Seventy-five of the 78 isolates (96.2%) carried at least two adhesin genes and two iron capture system genes. Evaluation of the biofilm formation capacity revealed that all (29/29) hospital-acquired isolates were biofilm producers with (6/29; 20.7%) strong biofilm producers, (15/29; 51.7%) moderate biofilm producers and (8/29; 27.6%) weak biofilm producers. Hospital-acquired isolates carrying papC had a greater biofilm formation capacity than those lacking papC (p < 0.001). The deepening of this type of study on bacterial virulence and hospital bacterial biofilms could lead to improvements in infection investigation, prevention, and therapeutic protocols.
},
year = {2023}
}
TY - JOUR T1 - High Prevalence of Virulence Genes and in Vitro Biofilm Production in Clinical Multidrug-Resistant Escherichia coli in Dakar Senegal AU - Komla Mawunyo Dossouvi AU - Bissoume Sambe Ba AU - Gora Lo AU - Issa Ndiaye AU - Awa Ba-Diallo AU - Ousmane Sow AU - Assane Dieng AU - Fatimata Talla AU - Abdoulaye Cissé AU - Serigne Mbaye Lo Ndiaye AU - Alioune Tine AU - Farba Karam AU - Habsa Diagne-Samb AU - Safietou Ngom-Cisse AU - Halimatou Diop-Ndiaye AU - Issaka Maman AU - Coumba Toure-Kane AU - Aïssatou Gaye-Diallo AU - Sika Dossim AU - Souleymane Mboup AU - Cheikh Saad Bouh Boye AU - Abdoulaye Seck AU - Makhtar Camara Y1 - 2023/10/31 PY - 2023 N1 - https://doi.org/10.11648/j.ijmb.20230804.11 DO - 10.11648/j.ijmb.20230804.11 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 69 EP - 81 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20230804.11 AB - Bacterial virulence is a key factor determining the outcome of each bacterial infection, and virulent bacteria are often associated with high-risk infections. Thus, this study aimed to screen for virulence genes and evaluate the in vitro biofilm formation capacity of multidrug-resistant Escherichia coli isolated in Dakar. For the 16 virulence genes identified by standard polymerase chain reaction (PCR), all 78 ExPEC isolates carried at least four virulence genes. The prevalence of virulence genes was as follows: adhesin genes fimH (98.7%), mrkD (98.7%), papC (46.2%), afaC (9%), and sfa/focDE (1.3%); iron acquisition system genes entB (98.7%), fepA (98.7%), ybtS (93.6%), fyuA (91%), iucA (91%), iucB (91%), iutA (34.6), iroB (6.4%), iroN (6.4%), and toxin genes hlyA (10.3%) and cnf (1 & 2) (10.3%). Seventy-five of the 78 isolates (96.2%) carried at least two adhesin genes and two iron capture system genes. Evaluation of the biofilm formation capacity revealed that all (29/29) hospital-acquired isolates were biofilm producers with (6/29; 20.7%) strong biofilm producers, (15/29; 51.7%) moderate biofilm producers and (8/29; 27.6%) weak biofilm producers. Hospital-acquired isolates carrying papC had a greater biofilm formation capacity than those lacking papC (p < 0.001). The deepening of this type of study on bacterial virulence and hospital bacterial biofilms could lead to improvements in infection investigation, prevention, and therapeutic protocols. VL - 8 IS - 4 ER -
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