Volume 3, Issue 3, September 2018, Page: 71-78
Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar
Hanitrinisoa Harimisa Andriamafana, Department of Fundamental and Applied Biochemistry, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar; National Center of Environmental Research, Antananarivo, Madagascar
Yves Mong, National Center of Environmental Research, Antananarivo, Madagascar
Onja Andriambeloson, National Center of Environmental Research, Antananarivo, Madagascar
Christine Ravonizafy, National Center of Environmental Research, Antananarivo, Madagascar
Marson Raherimandimby, Department of Fundamental and Applied Biochemistry, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar
Rado Rasolomampianina, National Center of Environmental Research, Antananarivo, Madagascar
Received: Sep. 11, 2018;       Accepted: Sep. 21, 2018;       Published: Oct. 30, 2018
DOI: 10.11648/j.ijmb.20180303.12      View  149      Downloads  8
Abstract
Heavy metals contamination is now widespread in the nature. At higher concentration, heavy metals become toxic and disturb the ecosystem including soil microorganisms. To adapt to such constraints, some microorganisms have developed tolerance mechanisms. Indeed, in the environment, the resistance of microorganisms to heavy metal often promotes to antibiotic resistance. This work aims to isolate strains from soil samples collected in Andralanitra landfill, to test their tolerance to heavy metals, to identify tolerant strains and to verify their resistance to antibiotics. According to the dilution method, a total of 48 strains were obtained, 14 were isolated on PDA medium, 10 on Sabouraud agar medium, 10 strains on Mossel agar medium, 7 on AS1 medium, 5 strains on TSA medium and 2 strains with King B medium. Resistance test to heavy metals performed by the wells method showed that out of the 48 isolated strains, 26 were capable to grow in the presence of heavy metals (solution composed of copper, zinc, cadmium, chromium, nickel, lead) at different concentrations. The highest number of tolerant strains was recorded at the concentration of 100mg/L ≤ C ≤ 1000mg/L. Four (4) strains were tolerant to the heavy metals solution at a concentration between 100mg/L and 1500mg/L. The molecular identification of these four most resistant strains by 16S rDNA gene sequencing and ITS gene sequencing allowed to classify them as belonging to the genera Ochrobactrum pseudogrignonense, Arthrobacter nicotianae, Penicillium crustosum and Penicillium commune. The antibiotic sensitivity test using disc diffusion method on Mueller-Hinton agar revealed that Ochrobactrum pseudogrignonense and Penicillium commune were resistant to Trimethoprim, Arthrobacter nicotianae showed resistance to Trimethoprim and Ciprofloxacin, Penicillium crustosum was resistant to all tested antibiotics.
Keywords
Isolation, Characterization, Resistance, Heavy Metals, Antibiotics, Dumpsite, MADAGASCAR
To cite this article
Hanitrinisoa Harimisa Andriamafana, Yves Mong, Onja Andriambeloson, Christine Ravonizafy, Marson Raherimandimby, Rado Rasolomampianina, Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar, International Journal of Microbiology and Biotechnology. Vol. 3, No. 3, 2018, pp. 71-78. doi: 10.11648/j.ijmb.20180303.12
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Y. Li, J. H. Li and C. Deng, Occurrence, characteristics and leakage of polybrominated diphenyl ethers in leachate from municipal solid waste landfills in China. Environ. Pollut. 184, 2014, pp: 94–100.
[2]
Alexander Cogut, Open burning of waste: a global health disaster, R20 Regions of climate action, October 2016.
[3]
A. Bhattacharya and A. Gupta, Evaluation of Acinetobacter sp. B9 for Cr (VI) resistance and detoxification with potential application in bioremediation of heavy-metals-rich industrial wastewater. Environmental Science and Pollution Research, volume 20, 2013, pp: 6628-6637.
[4]
M. A. Ashraf, M. J. Maah and I. Yusoff, Chemical speciation and potential mobility of heavy metals in the soil of former Tin mining catchment, Scientific World Journal, 2012.
[5]
L. J. Piddock, Multidrug-resistance efflux pumps–not just for resistance. Nat Rev Microbiol, 2006, 4: 629–636.
[6]
M. Gomathy and K. G. Sabarinathan, Microbial mechanisms of heavy metal tolerance- a review, Agric. Rev., 31 (2), 2010, pp: 133 – 138.
[7]
A. Malik and A. Aleem, Incidence of metal and antibiotic resistance in Pseudomonas spp from the river water, agricultural soil irrigated with waste water and ground water. Environ. Monit. Assess, 2011.
[8]
T. Ntakirutimana, T. Gang Du, J. Guou, and L. Huang, Pollution and potential ecological risk assessment of heavy metals in a lake. Pol. J. Environ. Stud. Volume 22, 2013, 1129.
[9]
D. Garhwal, G. Vaghela, T. Panwala, S. Revdiwala, A. Shah and S. Mulla, Lead tolerance capacity of clinical bacterial isolates and change in their antibiotic susceptibility pattern after exposure to a heavy metal. International Journal of Medicine and Public Health, 2014, 4(3): 253-256.
[10]
A. O. Summers, Generally overlooked fundamentals of bacterial genetics and ecology. Clinical Infectious Diseases, 2002, 34 (Suppl 3):S85-92.
[11]
Z. Yu, L. Gunn, P. Wall and S. Fanning, Antimicrobial resistance and its association with tolerance to heavy metals in agriculture production, Food microbiology, Volume 64, June 2017, pp: 23-32.
[12]
J. C. Philip, R. M. Atlas, and C. J. Cunningham, Bioremediation in Nature Encyclopedia of Live Science, 2001, pp. 1-10.
[13]
Don B Clewell, Antibiotic Resistance Plasmids in Bacteria, Citable reviews in the life science, February 2014.
[14]
Institut National de la Statistique (INSTAT) Madagascar, 2014.
[15]
J. Rodier, Analyse de l’eau, eaux naturelles, eaux résiduaires, eau de mer. 7e ed. Dunod. Paris, 1984.
[16]
G. H. Holt, N. R. Krieg, P. H. A. Sneath, J. T. Staley, and S. T. Williams. Bergey‘s Manual of determinative Bacteriology, 9th edition, Lippincott Williams and Wilkins, Philadelphia, 2000.
[17]
S. Senthilkumar, K. Sivakumar, and L. Kannan, Mercury resistant halophilic Actinomycetes from the salt marsh environment of velar estuary, southeast coast of India. J. Aqua. Biol., 2005, 20: 141-145.
[18]
A. K. Yadav, R. Kumar, R. Saikia, T. C. Bora, and D. K. Arora, Novel copper resistant and antimicrobial Streptomyces isolated from Bay of Bengal, India. J. de myc. Med., 2009, 19:234-240.
[19]
F. Baquero, M. C. Negri, M. I. Morosini, and J. Blazquez, Antibiotic-selective environments. Clinical infectious Diseases, 1998, 27: S5-S11.
[20]
N. Soumitra, D. Bibhas, and S. Indu, Isolation and Characterization of Cadmium and Lead Resistant Bacteria. Global Advanced Research Journal of Microbiology, Vol. 1(11) 2012, pp: 194-198.
[21]
H. E. Mohamed, Multiple heavy metal and antibiotic resistance of Acinetobacter baumannii strain HAF– 13 isolated from industrial effluents. American Journal of Microbiological Research, Vol. 4, No. 1, 2016, pp: 26-36.
[22]
Q. Sadia, K. Ibrar, M. Farhana, Z. Yangguo, G. Qingbao and P. Changsheng, Isolation and characterization of heavy metal resistant fungal isolates from industrial soil in China. Pakistan journal of zoology, vol.48 (5), 2016, pp: 1241-1247.
[23]
N. Rijal, Potato Dextrose Agar (PDA): principle, composition and colony characteristics. Culture Media used in Microbiology, 2015.
[24]
A. Rabia, Tasneem, and A. Adam, Effect of heavy metals on soil microbial community and mung seed germination. Pak J Bot. 39, 2007. E. Abatenh, B. Gizaw, Z. Tsegaye and M. Wassie, The role of microorganisms in bioremediation- a review, Open Journal of Environmental Biology, 2017.
[25]
M. Hookoom and D. Puchooa, Isolation and identification of heavy metals tolerant bacteria from industrial and agricultural areas in Mauritius, Current Research in Microbiology and Biotechnology, Vol. 1, 2013, 3: 119-123.
[26]
R. A. Ansari, A. A. Qureshi, and D. S. Ramteke, Isolation and characterization of heavy-metal resistant microbes from Industrial soil, International journal of environmental sciences Volume 6, No 5, 2016.
[27]
M. S. Adebisi, O. A. Oluwatosin, and T. A. Olumayowa, Resistance of bacteria isolated from Awotan dumpsite leachate to heavy metals and selected antibiotics. International Journal of Research in Pharmacy and Biosciences, Volume 2, Issue 9, October 2015, pp: 8-17.
[28]
Y. Ma, R. S. Oliveira, H. Freitas and C. Zhang, Biochemical and molecular mechanisms of plant-microbe-metal intractions: relevance for phytoremediation, Frontier Plant Science, volume 7, 2016.
[29]
O. B. Ojuederie and O. O. Babalola, Microbial and plant-assisted bioremediation of heavy metal polluted environments: a review. International journal of environmental research and public health, volume 14, December 2017.
[30]
Y. Yang, X. Yu, and R. Zhang, Draft genome sequence of Ochrobactrum pseudogrignonense strain CDB2, a highly efficient arsenate-resistant soil bacterium from arsenic-contaminated cattle dip sites. Genome announcements, 2013. 1. 10.1128/ genome A. 00173-13.
[31]
P. Liao et al., Chromium-resistant endophytic bacteria from Deyeuxia scabrescens (Griseb.) in Chromium contaminated area: isolation, screening and plant growth promoting. Chinese Journal of Applied & Environmental Biology, 2015, pp: 1025-1031.
[32]
X. Yu et al., Culturable Heavy Metal-Resistant and Plant Growth Promoting Bacteria in V-Ti Magnetite Mine Tailing Soil from Panzhihua, China, PloS One, volume 9, 2014.
[33]
D. Ignacio et al., Cadmium biosorption properties of the metal‐resistant Ochrobactrumcytisi Azn6.2. Engineering in Life Sciences Volume 10, Issue 1, 2010, pp: 49–56.
[34]
D. Satarupa and A. K. Paul, In-vitro bioreduction of hexavalent chromium by viable whole cells of Arthrobacter sp. 1201, Journal of Microbiology, Biotechnology and Food Sciences, 2014, pp: 19-23.
[35]
B. Goutam, K. R. Arun, P. Shubhant, and K. Ravi, An alternative approach of toxic heavy metal removal by Arthrobacter phenanthrenivorans: assessment of surfactant production and oxidative stress. Current Science, vol. 110, 2016.
[36]
A. S. Ayangbenro and O. O. Babalola, A new strategy for heavy metal polluted environments: a review of microbial biosorbents, International journal of environmental research and public health, volume 14, January 2017.
[37]
Xu et al., Role of Penicillium chrysogenum XJ-1 in the detoxification and bioremediation of cadmium, frontiers in Microbiology, volume 6, 2015.
[38]
B. A. Oso, M. O. Olagunji and P. A. Okiki, Lead tolerance and bioadsorption potentials of indigenous soil fungi in Ado Ekiti, Nigeria. European Journal of Experimental Biology, 2015, pp: 15-19.
[39]
A. Bahobil, R. A. Bayoumi, H. M. Atta and M. M. El-Sehrawey, Fungal biosorption for cadmium and mercury heavy metal ions isolated from some polluted localities in KSA. International Journal of Current Microbiology and Applied Sciences, volume 6, 2017, pp: 2138-2154.
[40]
B. Volesky, Biosorption and me. Water Res., 2007
[41]
D. J. Kim, D. I. Lee, and J. Keller, Effect of temperature and FA on nitrification and nitrite accumulation in landfill leachate and analysis of its nitrifing bacterial community by FISH. Bioresource technology, 2006, pp: 459-468.
[42]
M. Cheesbrough, District Laboratory Practice in Tropical Countries (Part II). Cambridge University, 2004, pp: 50-150.
[43]
S. D. Roger, M. R. Bhave, J. F. Mercer, J. Camakaris, and B. T. Lee, Cloning and characterization of cut E, a gene involved in copper transport in Escherichia coli. J Bacteriol, 173, 2004, pp: 6742-6748.
[44]
A. Verma, S. A. Singh, N. R. Bishnoi and A. Gupta, Biosorption of Cu (II) using free and immobilized biomass of Penicillium citrinum. Ecol. Eng., 61(A), 2013, pp: 486–490.
[45]
J. C. Frisvad and O. Filtenborg, Terverticillate Penicillia: chemotaxonomy and mycotoxin production. Mycologia, 1989, 81: 837– 861.
[46]
A. A. Ismaiel, J. Papenbrock, The effects of patulin from Penicilllium vulpinum on seedling growth, root tip ultrastructure and glutathione content of maize. Eur. J. Plant Pathol. 2014, pp: 497–509.
[47]
Walter and L. Sean, Acute penitrem A and roquefortine poisoning in a dog, The Canadian Veterinary Journal, 2002-5, 43:372–374.
[48]
H. F. Berntsen, I. L. Bogen, M. B. Wigestrand, F. Fonnum, S. I. Walaas, A. Moldes-Anaya, The fungal neurotoxin penitrem A induces the production of reactive oxygen species in human neutrophils at submicromolarconcentration. Toxicology, 2017, 392: 64–70.
[49]
H. W. Hu et al., Field-based evidence for copper contamination induced changes of antibiotic resistance in agicultural soils. Environ Microbiol, 2016.
Browse journals by subject