Detection of <i>Picornavirales</i> Viruses Using Viral Metagenomics in Wild Boars (<i>Sus scrofa</i>) from Guangdong Province, China

Ping Liu; Jia Bin Zhou; Lin Miao Li; Wen Zhong Huang; Jin Ping Chen

doi:doi:10.11648/j.ijmb.20220701.13

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Detection of Picornavirales Viruses Using Viral Metagenomics in Wild Boars (Sus scrofa) from Guangdong Province, China

Ping Liu, Jia Bin Zhou, Lin Miao Li, Wen Zhong Huang, Jin Ping Chen

Published in International Journal of Microbiology and Biotechnology (Volume 7, Issue 1)

Received: 23 January 2022 Accepted: 9 February 2022 Published: 25 February 2022

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Abstract

Wild boars (Sus scrofa) are hosts of several viruses that result in highly destructive diseases. Identifying and cataloging viruses carried by wild boars is a logical approach to evaluate the range of potential viruses and help with the conservation of both wild boars and cultivated pigs, as well as human beings, considering the possibility of zoonotic virus transmission from pigs to humans. In this study, eight lung tissue samples and 11 mixtures of anal and pharyngeal swabs were collected from 11 healthy wild boar individuals. Viral metagenomic analyses were conducted to detect virus diversity in wild boars. 1,199 contigs from a total of 184,434 de novo assembled contigs were determined as viral sequences, of which 71 contigs had a high level of sequence similarity to known RNA viruses. The dominant viruses were Sakobuvirus A and Posavirus 1, both of which belong to the Picornavirales family. A draft genome of Sakobuvirus A, covering 80.6% of the genome of the feline Sakobuvirus A strain, as well as a whole genome sequence of a Posavirus 1 strain with a length of 9,226 bp were obtained. Although there is little information on the etiology and pathogenesis of these two Picornavirales strains, their detection will enrich the information in the Picornavirales database and further expedite Picornavirales research on genetic diversity, epidemiology, and evolution.

Published in	International Journal of Microbiology and Biotechnology (Volume 7, Issue 1)
DOI	10.11648/j.ijmb.20220701.13
Page(s)	16-30
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Virome, Sus scrofa, Picornaviridae, Posaviruses, Phylogeny

References

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APA Style

Ping Liu, Jia Bin Zhou, Lin Miao Li, Wen Zhong Huang, Jin Ping Chen. (2022). Detection of Picornavirales Viruses Using Viral Metagenomics in Wild Boars (Sus scrofa) from Guangdong Province, China. International Journal of Microbiology and Biotechnology, 7(1), 16-30. https://doi.org/10.11648/j.ijmb.20220701.13

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ACS Style

Ping Liu; Jia Bin Zhou; Lin Miao Li; Wen Zhong Huang; Jin Ping Chen. Detection of Picornavirales Viruses Using Viral Metagenomics in Wild Boars (Sus scrofa) from Guangdong Province, China. Int. J. Microbiol. Biotechnol. 2022, 7(1), 16-30. doi: 10.11648/j.ijmb.20220701.13

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AMA Style

Ping Liu, Jia Bin Zhou, Lin Miao Li, Wen Zhong Huang, Jin Ping Chen. Detection of Picornavirales Viruses Using Viral Metagenomics in Wild Boars (Sus scrofa) from Guangdong Province, China. Int J Microbiol Biotechnol. 2022;7(1):16-30. doi: 10.11648/j.ijmb.20220701.13

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@article{10.11648/j.ijmb.20220701.13,
  author = {Ping Liu and Jia Bin Zhou and Lin Miao Li and Wen Zhong Huang and Jin Ping Chen},
  title = {Detection of Picornavirales Viruses Using Viral Metagenomics in Wild Boars (Sus scrofa) from Guangdong Province, China},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {1},
  pages = {16-30},
  doi = {10.11648/j.ijmb.20220701.13},
  url = {https://doi.org/10.11648/j.ijmb.20220701.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220701.13},
  abstract = {Wild boars (Sus scrofa) are hosts of several viruses that result in highly destructive diseases. Identifying and cataloging viruses carried by wild boars is a logical approach to evaluate the range of potential viruses and help with the conservation of both wild boars and cultivated pigs, as well as human beings, considering the possibility of zoonotic virus transmission from pigs to humans. In this study, eight lung tissue samples and 11 mixtures of anal and pharyngeal swabs were collected from 11 healthy wild boar individuals. Viral metagenomic analyses were conducted to detect virus diversity in wild boars. 1,199 contigs from a total of 184,434 de novo assembled contigs were determined as viral sequences, of which 71 contigs had a high level of sequence similarity to known RNA viruses. The dominant viruses were Sakobuvirus A and Posavirus 1, both of which belong to the Picornavirales family. A draft genome of Sakobuvirus A, covering 80.6% of the genome of the feline Sakobuvirus A strain, as well as a whole genome sequence of a Posavirus 1 strain with a length of 9,226 bp were obtained. Although there is little information on the etiology and pathogenesis of these two Picornavirales strains, their detection will enrich the information in the Picornavirales database and further expedite Picornavirales research on genetic diversity, epidemiology, and evolution.},
 year = {2022}
}

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TY  - JOUR
T1  - Detection of Picornavirales Viruses Using Viral Metagenomics in Wild Boars (Sus scrofa) from Guangdong Province, China
AU  - Ping Liu
AU  - Jia Bin Zhou
AU  - Lin Miao Li
AU  - Wen Zhong Huang
AU  - Jin Ping Chen
Y1  - 2022/02/25
PY  - 2022
N1  - https://doi.org/10.11648/j.ijmb.20220701.13
DO  - 10.11648/j.ijmb.20220701.13
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 16
EP  - 30
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20220701.13
AB  - Wild boars (Sus scrofa) are hosts of several viruses that result in highly destructive diseases. Identifying and cataloging viruses carried by wild boars is a logical approach to evaluate the range of potential viruses and help with the conservation of both wild boars and cultivated pigs, as well as human beings, considering the possibility of zoonotic virus transmission from pigs to humans. In this study, eight lung tissue samples and 11 mixtures of anal and pharyngeal swabs were collected from 11 healthy wild boar individuals. Viral metagenomic analyses were conducted to detect virus diversity in wild boars. 1,199 contigs from a total of 184,434 de novo assembled contigs were determined as viral sequences, of which 71 contigs had a high level of sequence similarity to known RNA viruses. The dominant viruses were Sakobuvirus A and Posavirus 1, both of which belong to the Picornavirales family. A draft genome of Sakobuvirus A, covering 80.6% of the genome of the feline Sakobuvirus A strain, as well as a whole genome sequence of a Posavirus 1 strain with a length of 9,226 bp were obtained. Although there is little information on the etiology and pathogenesis of these two Picornavirales strains, their detection will enrich the information in the Picornavirales database and further expedite Picornavirales research on genetic diversity, epidemiology, and evolution.
VL  - 7
IS  - 1
ER  -

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Author Information

Ping Liu

Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou, China
Jia Bin Zhou

Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou, China
Lin Miao Li

Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou, China
Wen Zhong Huang

Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou, China
Jin Ping Chen

Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou, China

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Ping Liu, Jia Bin Zhou, Lin Miao Li, Wen Zhong Huang, Jin Ping Chen. (2022). Detection of Picornavirales Viruses Using Viral Metagenomics in Wild Boars (Sus scrofa) from Guangdong Province, China. International Journal of Microbiology and Biotechnology, 7(1), 16-30. https://doi.org/10.11648/j.ijmb.20220701.13

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ACS Style

Ping Liu; Jia Bin Zhou; Lin Miao Li; Wen Zhong Huang; Jin Ping Chen. Detection of Picornavirales Viruses Using Viral Metagenomics in Wild Boars (Sus scrofa) from Guangdong Province, China. Int. J. Microbiol. Biotechnol. 2022, 7(1), 16-30. doi: 10.11648/j.ijmb.20220701.13

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AMA Style

Ping Liu, Jia Bin Zhou, Lin Miao Li, Wen Zhong Huang, Jin Ping Chen. Detection of Picornavirales Viruses Using Viral Metagenomics in Wild Boars (Sus scrofa) from Guangdong Province, China. Int J Microbiol Biotechnol. 2022;7(1):16-30. doi: 10.11648/j.ijmb.20220701.13

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@article{10.11648/j.ijmb.20220701.13,
  author = {Ping Liu and Jia Bin Zhou and Lin Miao Li and Wen Zhong Huang and Jin Ping Chen},
  title = {Detection of Picornavirales Viruses Using Viral Metagenomics in Wild Boars (Sus scrofa) from Guangdong Province, China},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {1},
  pages = {16-30},
  doi = {10.11648/j.ijmb.20220701.13},
  url = {https://doi.org/10.11648/j.ijmb.20220701.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220701.13},
  abstract = {Wild boars (Sus scrofa) are hosts of several viruses that result in highly destructive diseases. Identifying and cataloging viruses carried by wild boars is a logical approach to evaluate the range of potential viruses and help with the conservation of both wild boars and cultivated pigs, as well as human beings, considering the possibility of zoonotic virus transmission from pigs to humans. In this study, eight lung tissue samples and 11 mixtures of anal and pharyngeal swabs were collected from 11 healthy wild boar individuals. Viral metagenomic analyses were conducted to detect virus diversity in wild boars. 1,199 contigs from a total of 184,434 de novo assembled contigs were determined as viral sequences, of which 71 contigs had a high level of sequence similarity to known RNA viruses. The dominant viruses were Sakobuvirus A and Posavirus 1, both of which belong to the Picornavirales family. A draft genome of Sakobuvirus A, covering 80.6% of the genome of the feline Sakobuvirus A strain, as well as a whole genome sequence of a Posavirus 1 strain with a length of 9,226 bp were obtained. Although there is little information on the etiology and pathogenesis of these two Picornavirales strains, their detection will enrich the information in the Picornavirales database and further expedite Picornavirales research on genetic diversity, epidemiology, and evolution.},
 year = {2022}
}

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TY  - JOUR
T1  - Detection of Picornavirales Viruses Using Viral Metagenomics in Wild Boars (Sus scrofa) from Guangdong Province, China
AU  - Ping Liu
AU  - Jia Bin Zhou
AU  - Lin Miao Li
AU  - Wen Zhong Huang
AU  - Jin Ping Chen
Y1  - 2022/02/25
PY  - 2022
N1  - https://doi.org/10.11648/j.ijmb.20220701.13
DO  - 10.11648/j.ijmb.20220701.13
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 16
EP  - 30
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20220701.13
AB  - Wild boars (Sus scrofa) are hosts of several viruses that result in highly destructive diseases. Identifying and cataloging viruses carried by wild boars is a logical approach to evaluate the range of potential viruses and help with the conservation of both wild boars and cultivated pigs, as well as human beings, considering the possibility of zoonotic virus transmission from pigs to humans. In this study, eight lung tissue samples and 11 mixtures of anal and pharyngeal swabs were collected from 11 healthy wild boar individuals. Viral metagenomic analyses were conducted to detect virus diversity in wild boars. 1,199 contigs from a total of 184,434 de novo assembled contigs were determined as viral sequences, of which 71 contigs had a high level of sequence similarity to known RNA viruses. The dominant viruses were Sakobuvirus A and Posavirus 1, both of which belong to the Picornavirales family. A draft genome of Sakobuvirus A, covering 80.6% of the genome of the feline Sakobuvirus A strain, as well as a whole genome sequence of a Posavirus 1 strain with a length of 9,226 bp were obtained. Although there is little information on the etiology and pathogenesis of these two Picornavirales strains, their detection will enrich the information in the Picornavirales database and further expedite Picornavirales research on genetic diversity, epidemiology, and evolution.
VL  - 7
IS  - 1
ER  -

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