Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions

Hongyan Bai; Jiachen Zhao; Chunna Ma; Hejiang Wei; Xiyan Li; Qiongqiong Fang; Peng Yang; Quanyi Wang; Dayan Wang; Li Xin

doi:doi:10.11648/j.ijmb.20220701.14

Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions

Hongyan Bai, Jiachen Zhao, Chunna Ma, Hejiang Wei, Xiyan Li, Qiongqiong Fang, Peng Yang, Quanyi Wang, Dayan Wang, Li Xin

Published in International Journal of Microbiology and Biotechnology (Volume 7, Issue 1)

Received: 24 December 2021 Accepted: 28 January 2022 Published: 9 March 2022

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Abstract

Isolation and propagation of influenza virus from Influenza Like Illness (ILI) clinical sample is essential for the surveillance of circulating virus, such as antigenic and genetic analyses, antiviral sensitivity surveillance, as well as annual influenza vaccine selection. Madin-Darby canine kidney (MDCK) cell is conventionally used for virus isolation in public health laboratories. Throat swap samples of Influenza like Illness (ILI) were collected from two sentinel hospitals and screened seasonal influenza by real-time reverse transcription polymerase chain reaction (RT–PCR). H3N2 positive samples were performed virus isolation in MDCK cells. Samples were stored under different conditions before inoculation, 1-2 days at 2-8°C, 4-5 day or 8-9 days at 2-8°C, and no less than two months at -80°C. The results showed that long term (>2 month) -80°C storage of clinical samples (15.12%) had significantly lower virus isolation rate compare to short term (1-2 days and 4-9 days) under 2-8°C storage (88.37% for 1-2 days and 52.33% for 4-9 days). For those samples stored at 4°C, the shorter of the storage time, the better of sample quality and virus activity could be obtained, resulting in higher isolation rate. This study provides evidence for influenza surveillance and sample quality control.

Published in	International Journal of Microbiology and Biotechnology (Volume 7, Issue 1)
DOI	10.11648/j.ijmb.20220701.14
Page(s)	31-36
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Influenza, Virus Isolation, Sample Quality, Sample Storage Condition

References

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APA Style

Hongyan Bai, Jiachen Zhao, Chunna Ma, Hejiang Wei, Xiyan Li, et al. (2022). Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions. International Journal of Microbiology and Biotechnology, 7(1), 31-36. https://doi.org/10.11648/j.ijmb.20220701.14

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ACS Style

Hongyan Bai; Jiachen Zhao; Chunna Ma; Hejiang Wei; Xiyan Li, et al. Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions. Int. J. Microbiol. Biotechnol. 2022, 7(1), 31-36. doi: 10.11648/j.ijmb.20220701.14

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AMA Style

Hongyan Bai, Jiachen Zhao, Chunna Ma, Hejiang Wei, Xiyan Li, et al. Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions. Int J Microbiol Biotechnol. 2022;7(1):31-36. doi: 10.11648/j.ijmb.20220701.14

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@article{10.11648/j.ijmb.20220701.14,
  author = {Hongyan Bai and Jiachen Zhao and Chunna Ma and Hejiang Wei and Xiyan Li and Qiongqiong Fang and Peng Yang and Quanyi Wang and Dayan Wang and Li Xin},
  title = {Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {1},
  pages = {31-36},
  doi = {10.11648/j.ijmb.20220701.14},
  url = {https://doi.org/10.11648/j.ijmb.20220701.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220701.14},
  abstract = {Isolation and propagation of influenza virus from Influenza Like Illness (ILI) clinical sample is essential for the surveillance of circulating virus, such as antigenic and genetic analyses, antiviral sensitivity surveillance, as well as annual influenza vaccine selection. Madin-Darby canine kidney (MDCK) cell is conventionally used for virus isolation in public health laboratories. Throat swap samples of Influenza like Illness (ILI) were collected from two sentinel hospitals and screened seasonal influenza by real-time reverse transcription polymerase chain reaction (RT–PCR). H3N2 positive samples were performed virus isolation in MDCK cells. Samples were stored under different conditions before inoculation, 1-2 days at 2-8°C, 4-5 day or 8-9 days at 2-8°C, and no less than two months at -80°C. The results showed that long term (>2 month) -80°C storage of clinical samples (15.12%) had significantly lower virus isolation rate compare to short term (1-2 days and 4-9 days) under 2-8°C storage (88.37% for 1-2 days and 52.33% for 4-9 days). For those samples stored at 4°C, the shorter of the storage time, the better of sample quality and virus activity could be obtained, resulting in higher isolation rate. This study provides evidence for influenza surveillance and sample quality control.},
 year = {2022}
}

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TY  - JOUR
T1  - Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions
AU  - Hongyan Bai
AU  - Jiachen Zhao
AU  - Chunna Ma
AU  - Hejiang Wei
AU  - Xiyan Li
AU  - Qiongqiong Fang
AU  - Peng Yang
AU  - Quanyi Wang
AU  - Dayan Wang
AU  - Li Xin
Y1  - 2022/03/09
PY  - 2022
N1  - https://doi.org/10.11648/j.ijmb.20220701.14
DO  - 10.11648/j.ijmb.20220701.14
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 31
EP  - 36
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20220701.14
AB  - Isolation and propagation of influenza virus from Influenza Like Illness (ILI) clinical sample is essential for the surveillance of circulating virus, such as antigenic and genetic analyses, antiviral sensitivity surveillance, as well as annual influenza vaccine selection. Madin-Darby canine kidney (MDCK) cell is conventionally used for virus isolation in public health laboratories. Throat swap samples of Influenza like Illness (ILI) were collected from two sentinel hospitals and screened seasonal influenza by real-time reverse transcription polymerase chain reaction (RT–PCR). H3N2 positive samples were performed virus isolation in MDCK cells. Samples were stored under different conditions before inoculation, 1-2 days at 2-8°C, 4-5 day or 8-9 days at 2-8°C, and no less than two months at -80°C. The results showed that long term (>2 month) -80°C storage of clinical samples (15.12%) had significantly lower virus isolation rate compare to short term (1-2 days and 4-9 days) under 2-8°C storage (88.37% for 1-2 days and 52.33% for 4-9 days). For those samples stored at 4°C, the shorter of the storage time, the better of sample quality and virus activity could be obtained, resulting in higher isolation rate. This study provides evidence for influenza surveillance and sample quality control.
VL  - 7
IS  - 1
ER  -

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Author Information

Hongyan Bai

Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Reference and Research on Influenza, Key Laboratory for Medical Virology and Viral Diseases, National Health Commission, Beijing, People's Republic of China
Jiachen Zhao

Beijing Center for Disease Prevention and Control, Beijing, People's Republic of China
Chunna Ma

Beijing Center for Disease Prevention and Control, Beijing, People's Republic of China
Hejiang Wei

Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Reference and Research on Influenza, Key Laboratory for Medical Virology and Viral Diseases, National Health Commission, Beijing, People's Republic of China
Xiyan Li

Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Reference and Research on Influenza, Key Laboratory for Medical Virology and Viral Diseases, National Health Commission, Beijing, People's Republic of China
Qiongqiong Fang

Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Reference and Research on Influenza, Key Laboratory for Medical Virology and Viral Diseases, National Health Commission, Beijing, People's Republic of China
Peng Yang

Beijing Center for Disease Prevention and Control, Beijing, People's Republic of China
Quanyi Wang

Beijing Center for Disease Prevention and Control, Beijing, People's Republic of China
Dayan Wang

Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Reference and Research on Influenza, Key Laboratory for Medical Virology and Viral Diseases, National Health Commission, Beijing, People's Republic of China
Li Xin

Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Reference and Research on Influenza, Key Laboratory for Medical Virology and Viral Diseases, National Health Commission, Beijing, People's Republic of China

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Plain Text BibTeX RIS

APA Style

Hongyan Bai, Jiachen Zhao, Chunna Ma, Hejiang Wei, Xiyan Li, et al. (2022). Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions. International Journal of Microbiology and Biotechnology, 7(1), 31-36. https://doi.org/10.11648/j.ijmb.20220701.14

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ACS Style

Hongyan Bai; Jiachen Zhao; Chunna Ma; Hejiang Wei; Xiyan Li, et al. Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions. Int. J. Microbiol. Biotechnol. 2022, 7(1), 31-36. doi: 10.11648/j.ijmb.20220701.14

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AMA Style

Hongyan Bai, Jiachen Zhao, Chunna Ma, Hejiang Wei, Xiyan Li, et al. Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions. Int J Microbiol Biotechnol. 2022;7(1):31-36. doi: 10.11648/j.ijmb.20220701.14

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@article{10.11648/j.ijmb.20220701.14,
  author = {Hongyan Bai and Jiachen Zhao and Chunna Ma and Hejiang Wei and Xiyan Li and Qiongqiong Fang and Peng Yang and Quanyi Wang and Dayan Wang and Li Xin},
  title = {Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {1},
  pages = {31-36},
  doi = {10.11648/j.ijmb.20220701.14},
  url = {https://doi.org/10.11648/j.ijmb.20220701.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220701.14},
  abstract = {Isolation and propagation of influenza virus from Influenza Like Illness (ILI) clinical sample is essential for the surveillance of circulating virus, such as antigenic and genetic analyses, antiviral sensitivity surveillance, as well as annual influenza vaccine selection. Madin-Darby canine kidney (MDCK) cell is conventionally used for virus isolation in public health laboratories. Throat swap samples of Influenza like Illness (ILI) were collected from two sentinel hospitals and screened seasonal influenza by real-time reverse transcription polymerase chain reaction (RT–PCR). H3N2 positive samples were performed virus isolation in MDCK cells. Samples were stored under different conditions before inoculation, 1-2 days at 2-8°C, 4-5 day or 8-9 days at 2-8°C, and no less than two months at -80°C. The results showed that long term (>2 month) -80°C storage of clinical samples (15.12%) had significantly lower virus isolation rate compare to short term (1-2 days and 4-9 days) under 2-8°C storage (88.37% for 1-2 days and 52.33% for 4-9 days). For those samples stored at 4°C, the shorter of the storage time, the better of sample quality and virus activity could be obtained, resulting in higher isolation rate. This study provides evidence for influenza surveillance and sample quality control.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions
AU  - Hongyan Bai
AU  - Jiachen Zhao
AU  - Chunna Ma
AU  - Hejiang Wei
AU  - Xiyan Li
AU  - Qiongqiong Fang
AU  - Peng Yang
AU  - Quanyi Wang
AU  - Dayan Wang
AU  - Li Xin
Y1  - 2022/03/09
PY  - 2022
N1  - https://doi.org/10.11648/j.ijmb.20220701.14
DO  - 10.11648/j.ijmb.20220701.14
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 31
EP  - 36
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20220701.14
AB  - Isolation and propagation of influenza virus from Influenza Like Illness (ILI) clinical sample is essential for the surveillance of circulating virus, such as antigenic and genetic analyses, antiviral sensitivity surveillance, as well as annual influenza vaccine selection. Madin-Darby canine kidney (MDCK) cell is conventionally used for virus isolation in public health laboratories. Throat swap samples of Influenza like Illness (ILI) were collected from two sentinel hospitals and screened seasonal influenza by real-time reverse transcription polymerase chain reaction (RT–PCR). H3N2 positive samples were performed virus isolation in MDCK cells. Samples were stored under different conditions before inoculation, 1-2 days at 2-8°C, 4-5 day or 8-9 days at 2-8°C, and no less than two months at -80°C. The results showed that long term (>2 month) -80°C storage of clinical samples (15.12%) had significantly lower virus isolation rate compare to short term (1-2 days and 4-9 days) under 2-8°C storage (88.37% for 1-2 days and 52.33% for 4-9 days). For those samples stored at 4°C, the shorter of the storage time, the better of sample quality and virus activity could be obtained, resulting in higher isolation rate. This study provides evidence for influenza surveillance and sample quality control.
VL  - 7
IS  - 1
ER  -

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