The demand for both homegrown and imported frozen beef sold through super shops and online sales channels in Bangladesh has increased in recent years. These frozen beef may harbor potential microbiological health hazards which are unexplored yet. Hence, the present investigative research was envisaged to assess the potential microbiological public health hazards lying in these frozen beef. A statistically valid 72 frozen beef samples from the 204 sales points were collected during June 2021 to April 2022. The beef samples were subjected to aerobic plate count (APC) to assess the total viable bacterial load. Prevalence of major frozen meat-borne pathogens Salmonella, Escherichia coli, Campylobacter, and Listeria monocytogenes were investigated. The pathogens were isolated following US Food and Drug Administration Bacteriological Analytical Manual, henceforth, confirmatory identification was made by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) technology. Real-time Polymerase Chain Reaction was used to detect pathogenic Escherichia coli. Out of 72 samples, 33 samples (46.0%) were found satisfactory, 32 samples (44.0%) were found marginal, and 7 samples (10.0%) were found unsatisfactory based on total viable bacterial load in frozen beef. Escherichia coli was identified in 63 samples (88%), but none of them was found pathogenic for humans. Based on the most probable number (MPN) of Escherichia coli, 49 samples (68%) were found satisfactory (< 50 MPN/g), 18 samples (25%) were found marginal (50 to 500 MPN/g), and 5 samples (7%) were found unsatisfactory (> 500 MPN/g). Salmonella was identified in two (2.7%), and Listeria was identified only in one (1.3%) sample. All the samples were found free from Campylobacter. Overall 93 ± 3% of frozen beef sold through different super shops in different areas in Bangladesh has been found safe for human consumption.
| Published in | International Journal of Microbiology and Biotechnology (Volume 7, Issue 2) |
| DOI | 10.11648/j.ijmb.20220702.18 |
| Page(s) | 98-105 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Beef, Quality, Salmonella, Escherichia coli, Campylobacter, Listeria
| [1] | Ercolini, D., Russo, F., Torrieri, E., Masi, P., and Villani, F. (2006) Changes in the spoilage-related microbiota of beef during refrigerated storage under different packaging conditions. Applied and Environmental Microbiology. 72 (7): 4663-4671. |
| [2] | Hafez, M., Ibrahim, H., and Amin, R. (2020) Assessment of Bacterial Evaluation of imported Frozen Meat. Benha Veterinary Medical Journal. 37 (2): 1-3. |
| [3] | Ncoko, P., Jaja, I. F., and Oguttu, J. W. (2020) Microbiological quality of beef, mutton, and water from different abattoirs in the Eastern Cape Province, South Africa. Veterinary world. 13 (7): 1363. |
| [4] | Food Standards New Zealand, A. (2018) Compendium of Microbiological Criteria for Food. https://www.foodstandards.gov.au/publications/Documents/Compedium%20of%20Microbiological%20Criteria/Compendium%20of%20Microbiological%20Criteria.pdf. [Retrieved on March 31, 2021]. |
| [5] | Biswas, A. K. and Mandal, P. K. (2016) Meat-borne Pathogens and Use of Natural Antimicrobials for Food Safety. Food Borne Pathogens and Antibiotic Resistance. |
| [6] | Islam, N., Tahsin, N., Tarrannum, N., Salihee, R. Z., Tarannum, S., and Sujana, J. T. M. Factors Influencing the Consumers’ Perceptions Towards Frozen and Ready-to-Cook Food Products in Bangladesh. in Proceedings of the 1st Global International Conference. 2019. |
| [7] | Intelligence, M. (2021) BANGLADESH FROZEN FOOD MARKET - GROWTH, TRENDS, COVID-19 IMPACT, AND FORECASTS (2021 - 2026). https://www.mordorintelligence.com/industry-reports/bangladesh-frozen-food-market. [Retrieved on March 10, 2021]. |
| [8] | DLS. (2021) Livestock Economy. Department of Livestock Services. http://www.dls.gov.bd/site/page/22b1143b-9323-44f8-bfd8-647087828c9b/Livestock-Economy [Retrieved on March 10, 2021]. |
| [9] | knoema. (2021) Bangladesh - Meat imports. https://knoema.com/atlas/Bangladesh/topics/Agriculture/Trade-Import-Value/Meat-imports#:~:text=In%202018%2C%20meat%20imports%20for,thousand%20US%20dollars%20in%202018. [Retrieved on March 10, 2021]. |
| [10] | Parvez, S. (2019) Foreign meat finding niche in local market. The Daily Star. https://www.thedailystar.net/business/news/foreign-meat-finding-niche-local-market-1805830 [Retrieved on March 10, 2021]. |
| [11] | Parvin, M., Talukder, S., Ali, M., Chowdhury, E. H., Rahman, M., and Islam, M. (2020) Antimicrobial Resistance Pattern of Escherichia coli Isolated from Frozen Chicken Meat in Bangladesh. Pathogens. 9 (6): 420. |
| [12] | Alam, S. T. (2015) Antibiogram of pre-processed raw chicken meat from different supershops of Dhaka city, Bangladesh. J. Allied Health Sci. 2: 45–52. |
| [13] | Uddin, J., Hossain, K., Hossain, S., Saha, K., Jubyda, F. T., Haque, R., Billah, B., Talukder, A. A., Parvez, A. K., and Dey, S. K. (2019) Bacteriological assessments of foodborne pathogens in poultry meat at different super shops in Dhaka, Bangladesh. Italian journal of food safety. 8 (1): 6720. |
| [14] | Faruque, M. O., Mahmud, S., Munayem, M. A., Sultana, R., Molla, M. T., Ali, M. F., Wasim, M., Sarker, S., and Evamoni, F. Z. (2019) Bacteriological analysis and public health impact of broiler meat: a study on Nalitabari Paurosova, Sherpur, Bangladesh. Advances in Microbiology. 9 (07): 581. |
| [15] | Andrews, W. H. and Hammack, T. S. (2003) Bacteriological Analytical Manual (BAM) Chapter 1: Food sampling and preparation of sample homogenate. Bacteriological Analytical Manual, US Food and Drug Administration. https://www.fda.gov/food/laboratory-methods-food/bam-chapter-1-food-samplingpreparation-sample-homogenate [Retrieved on March 15, 2021]. |
| [16] | Maturin, L. and Peeler, J. (2001) Bacteriological Analytical Manual (BAM) Chapter 3: Aerobic plate count. Bacteriological analytical manual (BAM), US Food and Drug Administration. https://www.fda.gov/food/laboratory-methods-food/bam-chapter-3-aerobic-plate-count. [Retrieved on March 15, 2021]. |
| [17] | Feng, P., Weagant, S. D., Grant, M. A., Burkhardt, W., Shellfish, M., and Water, B. (2002) Bacteriological Analytical Manual (BAM) Chapter 4: Enumeration of Escherichia coli and the Coliform Bacteria. Bacteriological analytical manual (BAM), US Food and Drug Administration. https://www.fda.gov/food/laboratory-methods-food/bam-chapter-4-enumeration-escherichia-coli-and-coliform-bacteria. [Retrieved on March 15, 2021]. |
| [18] | Bastin, B., Bird, P., Benzinger, M. J., Crowley, E., Agin, J., Goins, D., Sohier, D., Timke, M., Shi, G., and Kostrzewa, M. (2018) Confirmation and Identification of Salmonella spp., Cronobacter spp., and Other Gram-Negative Organisms by the Bruker MALDI Biotyper Method: Collaborative Study, First Action 2017.09. Journal of AOAC International. 101 (5): 1593-1609. |
| [19] | Verdure, C. (2008) The (EC) Regulation on microbiological Criteria: a general Overview. European Food and Feed Law Review. 3 (3): 127-177. http://www.jstor.org/stable/24325282. |
| [20] | Andrews, W. H., Jacobson, A., and Hammack, T. (2011) Bacteriological Analytical Manual (BAM) chapter 5: Salmonella. Bacteriological Analytical Manual, US Food and Drug Administration. https://www.fda.gov/food/laboratory-methods-food/bam-chapter-5-salmonella. [Retrieved on March 15, 2021]. |
| [21] | Hunt, J. M., Abeyta, C., and Tran, T. (2018) Bacteriological Analytical Manual (BAM) Chapter 7: Campylobacter. Bacteriological Analytical Manual (BAM), US Food and Drug Administration. https://www.fda.gov/food/laboratory-methods-food/bam-chapter-7-campylobacter [Retrieved on March 15, 2021]. |
| [22] | Hitchins, A. D., Jinneman, K., and Chen, Y. (2004) Bacteriological Analytical Manual (BAM) Chapter 10: Detection of Listeria monocytogenes in foods and environmental samples, and enumeration of Listeria monocytogenes in foods.. Bacteriological Analytical Manual, US Food and Drug Administration. https://www.fda.gov/food/laboratory-methods-food/bam-chapter-10-detection-listeria-monocytogenes-foods-and-environmental-samples-and-enumeration [Retrieved on March 15, 2021]. |
| [23] | Kang, L., Li, N., Li, P., Zhou, Y., Gao, S., Gao, H., Xin, W., and Wang, J. (2017) MALDI-TOF mass spectrometry provides high accuracy in identification of Salmonella at species level but is limited to type or subtype Salmonella serovars. European journal of mass spectrometry. 23 (2): 70-82. |
| [24] | Conrad, C. C., Stanford, K., McAllister, T. A., Thomas, J., and Reuter, T. (2014) Further development of sample preparation and detection methods for O157 and the top 6 non-O157 STEC serogroups in cattle feces. Journal of Microbiological Methods. 105: 22-30. |
| [25] | Karmali, M. A. (2017) Emerging public health challenges of Shiga toxin–producing Escherichia coli related to changes in the pathogen, the population, and the environment. Clinical Infectious Diseases. 64 (3): 371-376. |
| [26] | Manage, D. P., Lauzon, J., Jones, C. M., Ward, P. J., Pilarski, L. M., Pilarski, P. M., and McMullen, L. M. (2019) Detection of pathogenic Escherichia coli on potentially contaminated beef carcasses using cassette PCR and conventional PCR. BMC Microbiology. 19 (1): 1-11. |
| [27] | Molina, F., López-Acedo, E., Tabla, R., Roa, I., Gómez, A., and Rebollo, J. E. (2015) Improved detection of Escherichia coli and coliform bacteria by multiplex PCR. BMC Biotechnology. 15 (1): 1-9. |
| [28] | Parvin, M., Ali, M., Talukder, S., Nahar, A., Chowdhury, E. H., Rahman, M., and Islam, M. (2021) Prevalence and multidrug resistance pattern of methicillin resistant S. aureus isolated from frozen chicken meat in Bangladesh. Microorganisms. 9 (3): 636. |
| [29] | Sharma, G. (2017) Pros and cons of different sampling techniques. International journal of applied research. 3 (7): 749-752. |
| [30] | Kilgannon, A. K., Holman, B. W., Mawson, A. J., Campbell, M., Collins, D., and Hopkins, D. L. (2019) The effect of different temperature-time combinations when ageing beef: Sensory quality traits and microbial loads. Meat science. 150: 23-32. |
| [31] | Lyautey, E., Lu, Z., Lapen, D. R., Wilkes, G., Scott, A., Berkers, T., Edge, T. A., and Topp, E. (2010) Distribution and diversity of Escherichia coli populations in the South Nation River drainage basin, eastern Ontario, Canada. Applied and Environmental Microbiology. 76 (5): 1486-1496. |
| [32] | Doyle, M. P. (1991) Escherichia coli O157: H7 and its significance in foods. International Journal of Food Microbiology. 12 (4): 289-301. |
| [33] | Franz, E., Klerks, M. M., De Vos, O. J., Termorshuizen, A. J., and van Bruggen, A. H. (2007) Prevalence of Shiga toxin-producing Escherichia coli stx 1, stx 2, eaeA, and rfbE genes and survival of E. coli O157: H7 in manure from organic and low-input conventional dairy farms. Applied and Environmental Microbiology. 73 (7): 2180-2190. |
| [34] | Otaguiri, E. S., Morguette, A. E. B., Morey, A. T., Tavares, E. R., Kerbauy, G., de Almeida Torres, R. S., Chaves Júnior, M., Tognim, M. C. B., Góes, V. M., and Krieger, M. A. (2018) Development of a melting-curve based multiplex real-time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides. BMC Pregnancy and Childbirth. 18 (1): 1-11. |
| [35] | Dong, P., Zhu, L., Mao, Y., Liang, R., Niu, L., Zhang, Y., Li, K., and Luo, X. (2014) Prevalence and profile of Salmonella from samples along the production line in Chinese beef processing plants. Food Control. 38: 54-60. |
APA Style
Md. Al-Amin, Md. Mizanur Rahman, Md. Mostofa Kamal. (2022). Microbiological Quality Assessment of Frozen Beef in Bangladesh. International Journal of Microbiology and Biotechnology, 7(2), 98-105. https://doi.org/10.11648/j.ijmb.20220702.18
ACS Style
Md. Al-Amin; Md. Mizanur Rahman; Md. Mostofa Kamal. Microbiological Quality Assessment of Frozen Beef in Bangladesh. Int. J. Microbiol. Biotechnol. 2022, 7(2), 98-105. doi: 10.11648/j.ijmb.20220702.18
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ijmb.20220702.18,
author = {Md. Al-Amin and Md. Mizanur Rahman and Md. Mostofa Kamal},
title = {Microbiological Quality Assessment of Frozen Beef in Bangladesh},
journal = {International Journal of Microbiology and Biotechnology},
volume = {7},
number = {2},
pages = {98-105},
doi = {10.11648/j.ijmb.20220702.18},
url = {https://doi.org/10.11648/j.ijmb.20220702.18},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220702.18},
abstract = {The demand for both homegrown and imported frozen beef sold through super shops and online sales channels in Bangladesh has increased in recent years. These frozen beef may harbor potential microbiological health hazards which are unexplored yet. Hence, the present investigative research was envisaged to assess the potential microbiological public health hazards lying in these frozen beef. A statistically valid 72 frozen beef samples from the 204 sales points were collected during June 2021 to April 2022. The beef samples were subjected to aerobic plate count (APC) to assess the total viable bacterial load. Prevalence of major frozen meat-borne pathogens Salmonella, Escherichia coli, Campylobacter, and Listeria monocytogenes were investigated. The pathogens were isolated following US Food and Drug Administration Bacteriological Analytical Manual, henceforth, confirmatory identification was made by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) technology. Real-time Polymerase Chain Reaction was used to detect pathogenic Escherichia coli. Out of 72 samples, 33 samples (46.0%) were found satisfactory, 32 samples (44.0%) were found marginal, and 7 samples (10.0%) were found unsatisfactory based on total viable bacterial load in frozen beef. Escherichia coli was identified in 63 samples (88%), but none of them was found pathogenic for humans. Based on the most probable number (MPN) of Escherichia coli, 49 samples (68%) were found satisfactory ( 500 MPN/g). Salmonella was identified in two (2.7%), and Listeria was identified only in one (1.3%) sample. All the samples were found free from Campylobacter. Overall 93 ± 3% of frozen beef sold through different super shops in different areas in Bangladesh has been found safe for human consumption.},
year = {2022}
}
TY - JOUR T1 - Microbiological Quality Assessment of Frozen Beef in Bangladesh AU - Md. Al-Amin AU - Md. Mizanur Rahman AU - Md. Mostofa Kamal Y1 - 2022/06/30 PY - 2022 N1 - https://doi.org/10.11648/j.ijmb.20220702.18 DO - 10.11648/j.ijmb.20220702.18 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 98 EP - 105 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20220702.18 AB - The demand for both homegrown and imported frozen beef sold through super shops and online sales channels in Bangladesh has increased in recent years. These frozen beef may harbor potential microbiological health hazards which are unexplored yet. Hence, the present investigative research was envisaged to assess the potential microbiological public health hazards lying in these frozen beef. A statistically valid 72 frozen beef samples from the 204 sales points were collected during June 2021 to April 2022. The beef samples were subjected to aerobic plate count (APC) to assess the total viable bacterial load. Prevalence of major frozen meat-borne pathogens Salmonella, Escherichia coli, Campylobacter, and Listeria monocytogenes were investigated. The pathogens were isolated following US Food and Drug Administration Bacteriological Analytical Manual, henceforth, confirmatory identification was made by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) technology. Real-time Polymerase Chain Reaction was used to detect pathogenic Escherichia coli. Out of 72 samples, 33 samples (46.0%) were found satisfactory, 32 samples (44.0%) were found marginal, and 7 samples (10.0%) were found unsatisfactory based on total viable bacterial load in frozen beef. Escherichia coli was identified in 63 samples (88%), but none of them was found pathogenic for humans. Based on the most probable number (MPN) of Escherichia coli, 49 samples (68%) were found satisfactory ( 500 MPN/g). Salmonella was identified in two (2.7%), and Listeria was identified only in one (1.3%) sample. All the samples were found free from Campylobacter. Overall 93 ± 3% of frozen beef sold through different super shops in different areas in Bangladesh has been found safe for human consumption. VL - 7 IS - 2 ER -
Email: